Detection of toxigenic Vibrio cholerae with new multiplex PCR
نویسندگان
چکیده
منابع مشابه
simultaneous detection of escherichia coli o157:h7, toxigenic vibrio cholerae, and salmonella typhimurium by multiplex pcr
background escherichia coli o157:h7, vibrio cholerae, and salmonella typhimurium are pathogenic bacteria found in contaminated water and food. no assay method is currently available on simultaneous detection or identification of all the three pathogens. our aim was to develop a rapid and reliable method for this purpose. materials and methods a protocol for sample collection, and a pcr procedur...
متن کاملDevelopment and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139.
A multiplex polymerase chain reaction assay was developed for concurrent detection of rfb sequences specific for the O1 and the O139 serogroups of Vibrio cholerae and for ctxA specific sequences. The multiplex PCR assay was found to be highly specific and sensitive and was capable of detecting 65 cfu and 200 cfu per assay of V. cholerae O1 and O139, respectively. Evaluation of the multiplex PCR...
متن کاملDetection and confirmation of toxigenic Vibrio cholerae O1 in environmental and clinical samples by a direct cell multiplex PCR
Epidemic cholera caused by toxigenic Vibrio cholerae O1 is a major health problem in several developing countries. Traditional methods for identifying V. cholerae involve cultural, biochemical and immunological assays which are cumbersome and often take several days to complete. In the present study, a direct cell multiplex PCR was developed targeting the ompW, ctxB and rfbO1 genes for confirma...
متن کاملRapid Screening of Toxigenic Vibrio cholerae O1 Strains from South Iran by PCR-ELISA
Background: The ability to sensitively detect Vibrio cholera with PCR-ELISA method represents a considerable advancement over alternative more time-consuming methods for detection of this pathogen. The aim of this research is to evaluate the suitability of a PCR-enzyme-linked immunosorbent assay for sensitive and rapid detection of V. cholera O1. Methods: The 398-bp sequence of a gene that cod...
متن کاملPreventing Maritime Transfer of Toxigenic Vibrio cholerae
Organisms, including Vibrio cholerae, can be transferred between harbors in the ballast water of ships. Zones in the Caribbean region where distance from shore and water depth meet International Maritime Organization guidelines for ballast water exchange are extremely limited. Use of ballast water treatment systems could mitigate the risk for organism transfer.
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ژورنال
عنوان ژورنال: Journal of Infection and Public Health
سال: 2012
ISSN: 1876-0341
DOI: 10.1016/j.jiph.2012.02.004